Hotspot

psbC-trnS-UGA

LSC · 37915-38167 · 253 bp

Back to Microtropis

Pi 0.0364

MarkerSeek score 68.60

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 253
variable_sites: 9
pi: 0.0364
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	GCTGCGGGATTTGAAAAAGG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.3	58.1	393-399 bp	1.0	80.3
psbC-trnS-UGA_p2	GCTGCGGGATTTGAAAAAGG	GATGGCCGAGTGGTTGATAG	50.0	55.0	58.3	58.1	404-410 bp	1.0	79.6
psbC-trnS-UGA_p3	TGCTGCGGGATTTGAAAAAG	GGTTGATAGCTCCGGTCTTG	45.0	55.0	57.6	58.1	394-400 bp	1.0	79.1
psbC-trnS-UGA_p4	CTGCTGCGGGATTTGAAAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	57.6	58.1	395-401 bp	1.0	79.0
psbC-trnS-UGA_p5	TAGGTCATTTATGGCACGCG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.4	58.1	431-437 bp	1.0	78.9