Hotspot

trnR-UCU-atpA

LSC · 10546-10835 · 290 bp

Back to Microtropis

Pi 0.0138

MarkerSeek score 50.00

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 290
variable_sites: 4
pi: 0.0138
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	TGGAATGAAAAGCGTCCATTG	TGGAACGTTTTCTACTTCAGGA	42.9	40.9	57.4	57.3	405-415 bp	1.0	46.8
trnR-UCU-atpA_p2	GGAATGAAAAGCGTCCATTGT	TGGAACGTTTTCTACTTCAGGA	42.9	40.9	57.4	57.3	404-414 bp	1.0	46.8
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	TGGAACGTTTTCTACTTCAGGA	47.6	40.9	57.3	57.3	344-354 bp	1.0	46.0
trnR-UCU-atpA_p4	TGGAATGAAAAGCGTCCATTG	ACGTTTTCTACTTCAGGAACAGT	42.9	39.1	57.4	58.0	401-411 bp	1.0	44.0
trnR-UCU-atpA_p5	GGAATGAAAAGCGTCCATTGT	ACGTTTTCTACTTCAGGAACAGT	42.9	39.1	57.4	58.0	400-410 bp	1.0	44.0