Hotspot

trnG-UCC-trnR-UCU

LSC · 10069-10473 · 405 bp

Back to Microtropis

Pi 0.0167

MarkerSeek score 65.50

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 405
variable_sites: 6
pi: 0.0167
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	ATAAATTGCGTCCATGCGTC	50.0	45.0	58.0	57.2	496-535 bp	1.0	78.6
trnG-UCC-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	ATAAATTGCGTCCATGCGTC	55.0	45.0	58.1	57.2	499-538 bp	1.0	78.4
trnG-UCC-trnR-UCU_p3	CCCTAGCCTTCCAAGCTAAC	ATAAATTGCGTCCATGCGTC	55.0	45.0	57.7	57.2	500-539 bp	1.0	76.2
trnG-UCC-trnR-UCU_p4	AGCCTTCCAAGCTAACGATG	AATAAATTGCGTCCATGCGTC	50.0	42.9	58.0	57.9	497-536 bp	1.0	76.2
trnG-UCC-trnR-UCU_p5	CCTAGCCTTCCAAGCTAACG	AATAAATTGCGTCCATGCGTC	55.0	42.9	58.1	57.9	500-539 bp	1.0	75.9