Hotspot

trnS-GCU-trnG-UCC

LSC · 8283-9212 · 930 bp

Back to Microtropis

Pi 0.0132

MarkerSeek score 65.30

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 930
variable_sites: 12
pi: 0.0132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CCGACGCTTTAATCCACTCA	TCAAGGGGATCACTAGAACGA	50.0	47.6	58.0	57.9	1005-1012 bp	1.0	74.0
trnS-GCU-trnG-UCC_p2	GCAACGGAAAGAGAGGGATT	TCAAGGGGATCACTAGAACGA	50.0	47.6	57.9	57.9	1066-1073 bp	1.0	72.9
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	TCAAGGGGATCACTAGAACGA	50.0	47.6	58.2	57.9	1063-1070 bp	1.0	72.6
trnS-GCU-trnG-UCC_p4	ATCAACGCAACGGAAAGAGA	TCAAGGGGATCACTAGAACGA	45.0	47.6	57.8	57.9	1072-1079 bp	1.0	72.5
trnS-GCU-trnG-UCC_p5	CCAAGTTATCAACGCAACGG	TCAAGGGGATCACTAGAACGA	50.0	47.6	57.8	57.9	1079-1086 bp	1.0	71.9