Hotspot

psbI-trnS-GCU

LSC · 7754-8194 · 441 bp

Back to Microtropis

Pi 0.0898

MarkerSeek score 82.70

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 441
variable_sites: 37
pi: 0.0898
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	GAAATCCCGGACGTGAAGAA	GTTCTTCGTACCGAGGGTTC	50.0	55.0	57.9	58.0	474-501 bp	1.0	89.3
psbI-trnS-GCU_p2	GAAATCCCGGACGTGAAGAA	TGAGTGGATTAAAGCGTCGG	50.0	50.0	57.9	58.0	514-541 bp	1.0	88.9
psbI-trnS-GCU_p3	GAAATCCCGGACGTGAAGAA	ATTGGGAGAGATGGCTGAGT	50.0	50.0	57.9	58.1	529-556 bp	1.0	87.9
psbI-trnS-GCU_p4	GAAATCCCGGACGTGAAGAA	GGCTGAGTGGATTAAAGCGT	50.0	50.0	57.9	58.3	517-544 bp	1.0	86.8
psbI-trnS-GCU_p5	GAAATCCCGGACGTGAAGAA	TGGCTGAGTGGATTAAAGCG	50.0	50.0	57.9	58.3	518-545 bp	1.0	86.8