Hotspot

ndhG-ndhI

SSC · 124184-124633 · 450 bp

Back to Micromeles

Pi 0.00156

MarkerSeek score 60.80

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 450
variable_sites: 1
pi: 0.00156
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	AATCATGTATTGGTCCGGGC	TCAAATTGCTTTGGGTCGTT	50.0	40.0	58.0	56.4	531-566 bp	1.0	71.5
ndhG-ndhI_p2	AATACCACCCCCATACCTCC	TCAAATTGCTTTGGGTCGTT	55.0	40.0	58.2	56.4	586-621 bp	1.0	70.3
ndhG-ndhI_p3	TGTATTGGTCCGGGCAAATC	TCAAATTGCTTTGGGTCGTT	50.0	40.0	58.2	56.4	526-561 bp	1.0	69.8
ndhG-ndhI_p4	AATCATGTATTGGTCCGGGC	ATCAAATTGCTTTGGGTCGT	50.0	40.0	58.0	56.2	532-567 bp	1.0	69.7
ndhG-ndhI_p5	AATCATGTATTGGTCCGGGC	TCAAATTGCTTTGGGTCGTTT	50.0	38.1	58.0	57.2	531-566 bp	1.0	69.5