Hotspot

rpl20-rps12

LSC · 72771-73541 · 771 bp

Back to Micromeles

Pi 0.00259

MarkerSeek score 60.60

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 771
variable_sites: 3
pi: 0.00259
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CTTGAATGAGCCCCTCGAAA	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	927-951 bp	1.0	75.1
rpl20-rps12_p2	TTGAATGAGCCCCTCGAAAG	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	926-950 bp	1.0	75.1
rpl20-rps12_p3	CTTGAATGAGCCCCTCGAAA	CAGCGACGAGGAACATGTAC	50.0	55.0	57.9	58.7	890-914 bp	1.0	73.6
rpl20-rps12_p4	TTGAATGAGCCCCTCGAAAG	CAGCGACGAGGAACATGTAC	50.0	55.0	57.9	58.7	889-913 bp	1.0	73.6
rpl20-rps12_p5	TTGAATGAGCCCCTCGAAAG	AGCGACGAGGAACATGTACT	50.0	50.0	57.9	58.8	888-912 bp	1.0	72.8