Hotspot

petA-psbJ

LSC · 66632-67611 · 980 bp

Back to Micromeles

Pi 0.0017

MarkerSeek score 59.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 980
variable_sites: 2
pi: 0.0017
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGCTCTTCTTGGCATCTGTT	AGCCGGTATTCTTGTGATCG	45.0	50.0	57.4	57.8	1150-1152 bp	1.0	73.8
petA-psbJ_p2	TTGCTCTTCTTGGCATCTGT	AGCCGGTATTCTTGTGATCG	45.0	50.0	57.4	57.8	1151-1153 bp	1.0	73.8
petA-psbJ_p3	CGTGTCCAAGGTCTTTTGCT	AGCCGGTATTCTTGTGATCG	50.0	50.0	58.7	57.8	1166-1168 bp	1.0	72.9
petA-psbJ_p4	GGTCTTTTGCTCTTCTTGGC	AGCCGGTATTCTTGTGATCG	50.0	50.0	57.3	57.8	1157-1159 bp	1.0	72.7
petA-psbJ_p5	TCCATTACGTGTCCAAGGTC	AGCCGGTATTCTTGTGATCG	50.0	50.0	57.2	57.8	1173-1175 bp	1.0	72.4