Hotspot

psbZ-trnG-GCC

LSC · 39128-39574 · 447 bp

Back to Micromeles

Pi 0.0135

MarkerSeek score 68.90

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 447
variable_sites: 6
pi: 0.0135
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTTGCTTCTCCTGAGGGTTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.7	57.8	587-605 bp	1.0	80.6
psbZ-trnG-GCC_p2	GGGTTGGTCGGGTAACAAAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.3	57.8	573-591 bp	1.0	80.4
psbZ-trnG-GCC_p3	TTCCGGCACATCATTATGGA	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.0	57.8	543-561 bp	1.0	75.0
psbZ-trnG-GCC_p4	AGGGTTGGTCGGGTAACAAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	59.2	57.8	574-592 bp	1.0	73.6
psbZ-trnG-GCC_p5	ATTTGCTTCTCCTGAGGGTTG	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	58.2	57.8	588-606 bp	1.0	73.2