Hotspot

trnK-UUU-rps16

LSC · 4253-5267 · 1015 bp

Back to Micromeles

Pi 0.000694
MarkerSeek score 63.60
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1015
variable_sites
1
pi
0.000694
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.95
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT CGGAACTTCGCCCTAATCAA 50.0 50.0 58.2 58.0 1047-1087 bp 1.0 82.6
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT CGGAACTTCGCCCTAATCAA 50.0 50.0 58.2 58.0 1046-1086 bp 1.0 82.6
trnK-UUU-rps16_p3 CCGCACTTAAAAGCCGAGTA CGGAACTTCGCCCTAATCAA 50.0 50.0 58.3 58.0 1056-1096 bp 1.0 81.7
trnK-UUU-rps16_p4 AAAGCCGAGTACTCTACCGT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 1102-1142 bp 1.0 81.2
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 1101-1141 bp 1.0 81.2