Hotspot

trnH-GUG-psbA

LSC · 102-362 · 261 bp

Back to Micromeles

Pi 0.0153

MarkerSeek score 54.60

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 261
variable_sites: 6
pi: 0.0153
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	ATATTATGGGCGAACGACGG	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	438-440 bp	1.0	80.2
trnH-GUG-psbA_p2	ATATTATGGGCGAACGACGG	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	388-390 bp	1.0	80.1
trnH-GUG-psbA_p3	ATATTATGGGCGAACGACGG	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	391-393 bp	1.0	80.0
trnH-GUG-psbA_p4	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	55.0	50.0	58.1	58.0	377-379 bp	1.0	80.0
trnH-GUG-psbA_p5	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	391-393 bp	1.0	79.9