Hotspot

ycf1

SSC · 121800-127361 · 5562 bp

Back to Micranthes

Pi 0.038

MarkerSeek score 73.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5562
variable_sites: 554
pi: 0.038
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GTTCGACGATAAGGCCCATT	AAAACCTCTTGTGACCCGTC	50.0	50.0	58.1	58.0	444-450 bp	1.0	87.8
ycf1_p2	GAACGAATCTGATCCCCCAC	AAAACCTCTTGTGACCCGTC	55.0	50.0	58.0	58.0	915-924 bp	0.666667	66.4
ycf1_p3	GTGAACGAATCTGATCCCCC	AAAACCTCTTGTGACCCGTC	55.0	50.0	58.0	58.0	917-926 bp	0.666667	66.4
ycf1_p4	GAACGAATCTGATCCCCCAC	GCAGAATACCGTCACCCATT	55.0	50.0	58.0	58.0	1306-1315 bp	0.666667	66.4
ycf1_p5	GTGAACGAATCTGATCCCCC	GCAGAATACCGTCACCCATT	55.0	50.0	58.0	58.0	1308-1317 bp	0.666667	66.4