Hotspot

ndhF

SSC · 108290-110527 · 2238 bp

Back to Micranthes

Pi 0.0883

MarkerSeek score 81.00

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2238
variable_sites: 549
pi: 0.0883
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AAAACCTCTTGTGACCCGTC	TTCATGGACTTCGTTCGGAA	50.0	45.0	58.0	57.2	2500-2591 bp	0.833333	74.1
ndhF_p2	AAAACCTCTTGTGACCCGTC	TTTCATGGACTTCGTTCGGA	50.0	45.0	58.0	57.2	2501-2592 bp	0.833333	74.1
ndhF_p3	GCAGAATACCGTCACCCATT	TTCATGGACTTCGTTCGGAA	50.0	45.0	58.0	57.2	2891-2982 bp	0.833333	73.7
ndhF_p4	GCAGAATACCGTCACCCATT	TTTCATGGACTTCGTTCGGA	50.0	45.0	58.0	57.2	2892-2983 bp	0.833333	73.7
ndhF_p5	CTTGTGACCCGTCTTTTCGA	TTCATGGACTTCGTTCGGAA	50.0	45.0	58.1	57.2	2493-2584 bp	0.833333	73.3