Hotspot

petG-trnW-CCA

LSC · 65166-65307 · 142 bp

Back to Micranthes

Pi 0.118

MarkerSeek score 82.10

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 142
variable_sites: 35
pi: 0.118
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	ACAGACGTGGTGATCAGTTG	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.8	58.0	236-246 bp	1.0	90.6
petG-trnW-CCA_p2	ACAGACGTGGTGATCAGTTG	AGAACGTGGGTCTCCAAAAC	50.0	50.0	57.8	58.0	214-224 bp	1.0	90.5
petG-trnW-CCA_p3	ACAGACGTGGTGATCAGTTG	CAAAACCCGATGTCGTAGGT	50.0	50.0	57.8	57.9	200-210 bp	1.0	90.0
petG-trnW-CCA_p4	ACAGACGTGGTGATCAGTTG	CGCTCTTAGTTCAGTTCGGT	50.0	50.0	57.8	57.7	234-244 bp	1.0	88.0
petG-trnW-CCA_p5	ACAGACGTGGTGATCAGTTG	CCCGATGTCGTAGGTTCAAA	50.0	50.0	57.8	57.6	195-205 bp	1.0	87.8