Hotspot

petA-psbJ

LSC · 61954-62684 · 731 bp

Back to Micranthes

Pi 0.0613

MarkerSeek score 83.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 731
variable_sites: 100
pi: 0.0613
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CAGGGCCAGAACTTCTTGTT	ATGTGGAGGGAAGTGGGTTA	50.0	50.0	58.0	57.9	944-1109 bp	1.0	91.5
petA-psbJ_p2	CAGGGCCAGAACTTCTTGTT	ACGGACTAGCTGTACCTACC	50.0	55.0	58.0	58.0	1290-1455 bp	1.0	90.5
petA-psbJ_p3	CAGGGCCAGAACTTCTTGTT	GGACTAGCTGTACCTACCGT	50.0	55.0	58.0	58.0	1288-1453 bp	1.0	90.5
petA-psbJ_p4	CAGGGCCAGAACTTCTTGTT	GAGCCGACAAGGAATTCCAT	50.0	50.0	58.0	58.0	1430-1595 bp	1.0	90.0
petA-psbJ_p5	CAGGGCCAGAACTTCTTGTT	TCCCTATTCATTGCGGGTTG	50.0	50.0	58.0	57.9	1519-1684 bp	1.0	89.6