Hotspot

trnE-UUC-trnT-GGU

LSC · 30297-30885 · 589 bp

Back to Micranthes

Pi 0.0464

MarkerSeek score 80.40

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 589
variable_sites: 66
pi: 0.0464
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	532-733 bp	1.0	90.7
trnE-UUC-trnT-GGU_p2	TTTCGTAGTACCCTACCCCC	ACCCGCTGTTCCAATTGAAT	55.0	45.0	57.8	58.1	855-1060 bp	1.0	89.4
trnE-UUC-trnT-GGU_p3	TTTCGTAGTACCCTACCCCC	AAAAGACCCGCTGTTCCAAT	55.0	45.0	57.8	58.0	860-1065 bp	0.833333	80.5
trnE-UUC-trnT-GGU_p4	TTTCGTAGTACCCTACCCCC	AAAGACCCGCTGTTCCAATT	55.0	45.0	57.8	58.0	859-1064 bp	0.833333	80.5
trnE-UUC-trnT-GGU_p5	CCAGCGTAGACATATTGCCA	AAAGACCCGCTGTTCCAATT	50.0	45.0	57.8	58.0	974-1179 bp	0.833333	79.4