Hotspot

trnK-UUU-rps16

LSC · 4338-4904 · 567 bp

Back to Micranthes

Pi 0.0381

MarkerSeek score 78.90

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 567
variable_sites: 58
pi: 0.0381
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	GAGAAGCCTTTCCCGCATTA	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.0	58.0	1052-1215 bp	0.833333	80.3
trnK-UUU-rps16_p2	AGAAGCCTTTCCCGCATTAG	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.0	58.0	1051-1214 bp	0.833333	80.3
trnK-UUU-rps16_p3	GAGAAGCCTTTCCCGCATTA	ACGAGACAAGAAGGGGGTTA	50.0	50.0	58.0	58.0	1476-1641 bp	0.833333	79.3
trnK-UUU-rps16_p4	AGAAGCCTTTCCCGCATTAG	ACGAGACAAGAAGGGGGTTA	50.0	50.0	58.0	58.0	1475-1640 bp	0.833333	79.3
trnK-UUU-rps16_p5	CCGATCCGGTAAGAATGCAA	ACGAGACAAGAAGGGGGTTA	50.0	50.0	58.1	58.0	1280-1445 bp	0.666667	67.3