Hotspot

atpE-atpB

Genome · 120300-120991 · 692 bp

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Pi 0.24
MarkerSeek score 75.00
Primer available yes
Label atpE-atpB

Feature Metadata

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpE-atpB_p1 TCATCTGCCTGATCATTCCA AAGCTGCGACACTATCATAG 45.0 45.0 56.3 55.1 774 bp 0.5 23.0
atpE-atpB_p2 TCATCTGCCTGATCATTCCA AAAGCTGCGACACTATCATAG 45.0 42.9 56.3 55.9 775 bp 0.5 21.4
atpE-atpB_p3 TCATCTGCCTGATCATTCCAA AAGCTGCGACACTATCATAG 42.9 45.0 57.1 55.1 774 bp 0.5 21.1
atpE-atpB_p4 TTCATCTGCCTGATCATTCCA AAGCTGCGACACTATCATAG 42.9 45.0 57.1 55.1 775 bp 0.5 21.1
atpE-atpB_p5 TCATCTGCCTGATCATTCCAA AAAGCTGCGACACTATCATAG 42.9 42.9 57.1 55.9 775 bp 0.5 20.0