Hotspot

trnH-GUG-trnL-CAA

Genome · 97543-98252 · 710 bp

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Pi 0.244

MarkerSeek score 83.10

Primer available yes

Label trnH-GUG-trnL-CAA

Feature Metadata

label_name: trnH-GUG-trnL-CAA
feature_type: igs
region: Genome
length_bp: 710
variable_sites: 161
pi: 0.244
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-trnL-CAA_p1	CAAGGCAGTGGATTGTGACT	GGTTTAAGCCCGATCAGTGA	50.0	50.0	58.1	57.6	1187-1617 bp	1.0	88.0
trnH-GUG-trnL-CAA_p2	CAAGGCAGTGGATTGTGACT	ACTGTATCGGTTTAAGCCCG	50.0	50.0	58.1	57.7	1195 bp	0.5	56.8
trnH-GUG-trnL-CAA_p3	GGATTGTGACTCCACCACTC	ACTGTATCGGTTTAAGCCCG	55.0	50.0	57.9	57.7	1186 bp	0.5	56.8
trnH-GUG-trnL-CAA_p4	GTCAAGGCAGTGGATTGTGA	ACTGTATCGGTTTAAGCCCG	50.0	50.0	58.1	57.7	1197 bp	0.5	56.7
trnH-GUG-trnL-CAA_p5	TCAAGGCAGTGGATTGTGAC	ACTGTATCGGTTTAAGCCCG	50.0	50.0	58.1	57.7	1196 bp	0.5	56.7