Hotspot

psbB-clpP

Genome · 45424-46414 · 991 bp

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Pi 0.169

MarkerSeek score 81.30

Primer available yes

Label psbB-clpP

Feature Metadata

label_name: psbB-clpP
feature_type: igs
region: Genome
length_bp: 991
variable_sites: 135
pi: 0.169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-clpP_p1	ACACGATACCATGGTAAACCC	CTTTAGGAACACCAATTGGCA	47.6	42.9	57.7	56.7	860-1036 bp	1.0	63.4
psbB-clpP_p2	ACACGATACCATGGTAAACCC	ACTTTAGGAACACCAATTGGC	47.6	42.9	57.7	56.7	861-1037 bp	1.0	63.4
psbB-clpP_p3	ACACGATACCATGGTAAACCC	ACTTTAGGAACACCAATTGGCA	47.6	40.9	57.7	58.4	861-1037 bp	1.0	62.4
psbB-clpP_p4	ACACGATACCATGGTAAACCC	TTTAGGAACACCAATTGGCA	47.6	40.0	57.7	55.4	859-1035 bp	1.0	61.3
psbB-clpP_p5	ACACGATACCATGGTAAACCC	CTTTAGGAACACCAATTGGCAT	47.6	40.9	57.7	57.2	860-1036 bp	1.0	59.4