Hotspot

trnS-UGA-psbM

Genome · 39873-40437 · 565 bp

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Pi 0.169

MarkerSeek score 87.80

Primer available yes

Label trnS-UGA-psbM

Feature Metadata

label_name: trnS-UGA-psbM
feature_type: igs
region: Genome
length_bp: 565
variable_sites: 89
pi: 0.169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 87.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbM_p1	TATTGGAAAGGTGGCAGAGC	CGCCGAGGATATTGACTTCC	50.0	55.0	57.9	58.2	678-860 bp	1.0	89.1
trnS-UGA-psbM_p2	TATTGGAAAGGTGGCAGAGC	CACGCCGAGGATATTGACTT	50.0	50.0	57.9	57.8	680-862 bp	1.0	89.0
trnS-UGA-psbM_p3	TAGTTGTGAGACTACCGGGG	CGCCGAGGATATTGACTTCC	55.0	55.0	58.2	58.2	628-810 bp	1.0	89.0
trnS-UGA-psbM_p4	TAGTTGTGAGACTACCGGGG	CACGCCGAGGATATTGACTT	55.0	50.0	58.2	57.8	630-812 bp	1.0	88.9
trnS-UGA-psbM_p5	TATTGGAAAGGTGGCAGAGC	TAAAGCAGTTGCAATCACGC	50.0	45.0	57.9	57.7	695-877 bp	1.0	88.1