Hotspot

rpl23-cemA

Genome · 29544-30361 · 818 bp

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Pi 0.123

MarkerSeek score 83.60

Primer available yes

Label rpl23-cemA

Feature Metadata

label_name: rpl23-cemA
feature_type: igs
region: Genome
length_bp: 818
variable_sites: 85
pi: 0.123
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23-cemA_p1	CGAGTAGCTTTTTCCGTACGA	CCTTCTACTGTAGCTACATATCA	47.6	39.1	58.1	54.5	910-927 bp	1.0	51.4
rpl23-cemA_p2	GCGGTTTTGTGAGTCTTACG	CCTTCTACTGTAGCTACATATCA	50.0	39.1	57.4	54.5	966-983 bp	1.0	51.0
rpl23-cemA_p3	GCGGTTTTGTGAGTCTTACG	CCTTCTACTGTAGCTACATATCAT	50.0	37.5	57.4	55.1	966-983 bp	1.0	51.0
rpl23-cemA_p4	TCGAATTTGCGGTTTTGTGAG	CCTTCTACTGTAGCTACATATCA	42.9	39.1	58.0	54.5	974-991 bp	1.0	50.9
rpl23-cemA_p5	TCGAATTTGCGGTTTTGTGA	CCTTCTACTGTAGCTACATATCA	40.0	39.1	56.9	54.5	974-991 bp	1.0	50.9