Hotspot

infA-rps8

Genome · 23285-23891 · 607 bp

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Pi 0.146

MarkerSeek score 84.00

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: Genome
length_bp: 607
variable_sites: 67
pi: 0.146
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.76
markerseek_score: 84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	ATTGAGTTACGACGCCTTCC	CGTCTTGGCGGAGAAATTCT	50.0	50.0	58.0	58.3	665-666 bp	1.0	89.8
infA-rps8_p2	ATTGAGTTACGACGCCTTCC	CTCGTCTTGGCGGAGAAATT	50.0	50.0	58.0	58.3	667-668 bp	1.0	89.8
infA-rps8_p3	ATTGAGTTACGACGCCTTCC	AAAATCTCGTCTTGGCGGAG	50.0	50.0	58.0	58.3	672-673 bp	1.0	89.7
infA-rps8_p4	ATTGAGTTACGACGCCTTCC	AGAAAATCTCGTCTTGGCGG	50.0	50.0	58.0	58.3	674-675 bp	1.0	89.7
infA-rps8_p5	ATTGAGTTACGACGCCTTCC	TCGTCTTGGCGGAGAAATTC	50.0	50.0	58.0	58.3	666-667 bp	1.0	89.7