Hotspot

rps11-rpl36

Genome · 21852-22615 · 764 bp

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Pi 0.162

MarkerSeek score 84.30

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: Genome
length_bp: 764
variable_sites: 113
pi: 0.162
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	ACCCTGATTTCTCGCATTCC	TAGTTTTGCCTGTGTGTCCC	50.0	50.0	58.0	58.0	1444-1480 bp	1.0	91.5
rps11-rpl36_p2	ACAAGTTTGCGCTGCATTTT	TAGTTTTGCCTGTGTGTCCC	40.0	50.0	58.1	58.0	1415-1451 bp	1.0	91.3
rps11-rpl36_p3	ACCCTGATTTCTCGCATTCC	GGAAGGCGTCGTAACTCAAT	50.0	50.0	58.0	58.0	1649-1685 bp	1.0	90.8
rps11-rpl36_p4	ACAAGTTTGCGCTGCATTTT	GGAAGGCGTCGTAACTCAAT	40.0	50.0	58.1	58.0	1620-1656 bp	1.0	90.8
rps11-rpl36_p5	ACGTTCACCCTGATTTCTCG	GGAAGGCGTCGTAACTCAAT	50.0	50.0	57.9	58.0	1655-1691 bp	1.0	90.6