Hotspot

rpoA-rps11

Genome · 20772-21458 · 687 bp

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Pi 0.172
MarkerSeek score 86.50
Primer available yes
Label rpoA-rps11

Feature Metadata

label_name
rpoA-rps11
feature_type
igs
region
Genome
length_bp
687
variable_sites
101
pi
0.172
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.85
markerseek_score
86.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoA-rps11_p1 ACCTTGCTCTTGAATTCGTGA CGAGACATTACTCCGATCCC 42.9 55.0 57.9 57.3 768-807 bp 1.0 77.3
rpoA-rps11_p2 ACCTTGCTCTTGAATTCGTGA CATTACTCCGATCCCACACA 42.9 50.0 57.9 57.0 763-802 bp 1.0 75.2
rpoA-rps11_p3 ACCTTGCTCTTGAATTCGTGA TACTCCGATCCCACACAATG 42.9 50.0 57.9 57.0 760-799 bp 1.0 75.2
rpoA-rps11_p4 ACCTTGCTCTTGAATTCGTGA ACATTACTCCGATCCCACAC 42.9 50.0 57.9 57.0 764-803 bp 1.0 75.1
rpoA-rps11_p5 TCGTGAATCCACACAAGAGA CGAGACATTACTCCGATCCC 45.0 55.0 56.5 57.3 753-792 bp 1.0 74.4