Hotspot

trnD-GUC-trnG-GCC

Genome · 126-857 · 732 bp

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Pi 0.231

MarkerSeek score 79.80

Primer available yes

Label trnD-GUC-trnG-GCC

Feature Metadata

label_name: trnD-GUC-trnG-GCC
feature_type: igs
region: Genome
length_bp: 732
variable_sites: 138
pi: 0.231
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnG-GCC_p1	TTCAATTGGTCAGAGCACCG	ACGCGGATTGTCACTAGTTC	50.0	50.0	58.5	58.0	925 bp	0.5	56.1
trnD-GUC-trnG-GCC_p2	TTCAATTGGTCAGAGCACCG	TTTGGGACCGGTACCTTTTG	50.0	50.0	58.5	58.0	1162 bp	0.5	56.1
trnD-GUC-trnG-GCC_p3	TTCAATTGGTCAGAGCACCG	GAGATTTTGGGACCGGTACC	50.0	55.0	58.5	58.0	1167 bp	0.5	55.9
trnD-GUC-trnG-GCC_p4	TTCAATTGGTCAGAGCACCG	GGCAAACCGGGTAATACCAT	50.0	50.0	58.5	57.9	1247 bp	0.5	55.7
trnD-GUC-trnG-GCC_p5	TTCAATTGGTCAGAGCACCG	CGAATCTAGTAGCAGGCACC	50.0	55.0	58.5	57.9	906 bp	0.5	55.0