Hotspot

ycf1

SSC · 118067-123538 · 5472 bp

Back to Metagentiana

Pi 0.00822

MarkerSeek score 48.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5472
variable_sites: 45
pi: 0.00822
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTTAAGCGGTATGGGCCATT	TCTTTTACATACCCACCCAGT	50.0	42.9	58.0	56.3	287 bp	1.0	59.3
ycf1_p2	TTAAGCGGTATGGGCCATTC	TCTTTTACATACCCACCCAGT	50.0	42.9	58.0	56.3	286 bp	1.0	59.2
ycf1_p3	GTTGTTCCAGGTACCGTCAG	TCTTTTACATACCCACCCAGT	55.0	42.9	58.2	56.3	237 bp	1.0	58.0
ycf1_p4	CTTAAGCGGTATGGGCCATT	ACATACCCACCCAGTTTATTCA	50.0	40.9	58.0	57.1	281 bp	1.0	57.8
ycf1_p5	TTAAGCGGTATGGGCCATTC	ACATACCCACCCAGTTTATTCA	50.0	40.9	58.0	57.1	280 bp	1.0	57.7