Hotspot

ndhF

SSC · 104953-107181 · 2229 bp

Back to Metagentiana

Pi 0.00538

MarkerSeek score 52.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2229
variable_sites: 12
pi: 0.00538
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AAGGGATCCACCGAAGAAGA	GAGGTAAGATCTGGTAAATCGGA	50.0	43.5	58.0	57.2	2344-2345 bp	1.0	49.7
ndhF_p2	AAGGGATCCACCGAAGAAGA	AGAGGTAAGATCTGGTAAATCGG	50.0	43.5	58.0	57.2	2345-2346 bp	1.0	49.7
ndhF_p3	CACAAAAGGGATCCACCGAA	GAGGTAAGATCTGGTAAATCGGA	50.0	43.5	58.1	57.2	2349-2350 bp	1.0	49.4
ndhF_p4	CACAAAAGGGATCCACCGAA	AGAGGTAAGATCTGGTAAATCGG	50.0	43.5	58.1	57.2	2350-2351 bp	1.0	49.3
ndhF_p5	AGGGATCCACCGAAGAAGAT	GAGGTAAGATCTGGTAAATCGGA	50.0	43.5	57.8	57.2	2343-2344 bp	1.0	48.7