Hotspot

ndhF-rpl32

SSC · 107182-107943 · 762 bp

Back to Metagentiana

Pi 0.00538

MarkerSeek score 64.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 762
variable_sites: 4
pi: 0.00538
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAGAAGACCCAACCCTAGGA	TAAAGCCGTCCAATACCCCT	50.0	50.0	57.3	58.4	920-930 bp	1.0	72.8
ndhF-rpl32_p2	AGAAGACCCAACCCTAGGAA	TAAAGCCGTCCAATACCCCT	50.0	50.0	57.3	58.4	919-929 bp	1.0	72.8
ndhF-rpl32_p3	AAGAAGACCCAACCCTAGGA	TTAAAGCCGTCCAATACCCC	50.0	50.0	57.3	57.6	921-931 bp	1.0	72.7
ndhF-rpl32_p4	AGAAGACCCAACCCTAGGAA	TTAAAGCCGTCCAATACCCC	50.0	50.0	57.3	57.6	920-930 bp	1.0	72.7
ndhF-rpl32_p5	AAGAAGACCCAACCCTAGGAA	TAAAGCCGTCCAATACCCCT	47.6	50.0	58.0	58.4	920-930 bp	1.0	70.7