Hotspot

infA-rps8

LSC · 75594-75664 · 71 bp

Back to Metagentiana

Pi 0.0282

MarkerSeek score 68.70

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 71
variable_sites: 2
pi: 0.0282
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	CCCACAAAACACAAAAGGGG	TTCTGTCCACTTCTCGAGGT	50.0	50.0	57.7	58.0	276 bp	1.0	78.0
infA-rps8_p2	GCCCTCGGGTTGAATCATAA	TTCTGTCCACTTCTCGAGGT	50.0	50.0	57.7	58.0	203 bp	1.0	78.0
infA-rps8_p3	GCCCTCGGGTTGAATCATAA	GAAGGAATCGGCGGAGAAAT	50.0	50.0	57.7	58.0	156 bp	1.0	77.9
infA-rps8_p4	CCCACAAAACACAAAAGGGG	GAAGGAATCGGCGGAGAAAT	50.0	50.0	57.7	58.0	229 bp	1.0	77.7
infA-rps8_p5	CCCACAAAACACAAAAGGGG	TAGAAGGAATCGGCGGAGAA	50.0	50.0	57.7	58.2	231 bp	1.0	76.3