Hotspot

petA-psbJ

LSC · 59606-60186 · 581 bp

Back to Metagentiana

Pi 0.00519

MarkerSeek score 61.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 581
variable_sites: 3
pi: 0.00519
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CCCATTACGTGTCCAAGGAC	CATATTCCGGATTGGGGTCA	55.0	50.0	58.3	57.1	728-743 bp	1.0	70.9
petA-psbJ_p2	GACCCATTACGTGTCCAAGG	CATATTCCGGATTGGGGTCA	55.0	50.0	58.3	57.1	730-745 bp	1.0	70.9
petA-psbJ_p3	ACGTGTCCAAGGACTTTTGT	CATATTCCGGATTGGGGTCA	45.0	50.0	57.6	57.1	722-737 bp	1.0	69.8
petA-psbJ_p4	CCCATTACGTGTCCAAGGAC	TGGTTCATATTCCGGATTGGG	55.0	47.6	58.3	57.8	733-748 bp	1.0	68.7
petA-psbJ_p5	GACCCATTACGTGTCCAAGG	TGGTTCATATTCCGGATTGGG	55.0	47.6	58.3	57.8	735-750 bp	1.0	68.7