Hotspot

accD-psaI

LSC · 55750-56158 · 409 bp

Back to Metagentiana

Pi 0.0176

MarkerSeek score 67.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 409
variable_sites: 7
pi: 0.0176
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TTCCACGCTTTCTTTCCCTT	ATTGCAGTTGCCGGAACTAA	45.0	45.0	57.6	58.1	525-707 bp	1.0	78.8
accD-psaI_p2	TTCCACGCTTTCTTTCCCTT	TTGCAGTTGCCGGAACTAAT	45.0	45.0	57.6	58.1	524-706 bp	1.0	78.8
accD-psaI_p3	AGTTCCACGCTTTCTTTCCC	TTGCAGTTGCCGGAACTAAT	50.0	45.0	58.4	58.1	526-708 bp	1.0	78.5
accD-psaI_p4	GTTCCACGCTTTCTTTCCCT	ATTGCAGTTGCCGGAACTAA	50.0	45.0	58.4	58.1	526-708 bp	1.0	78.5
accD-psaI_p5	GTTCCACGCTTTCTTTCCCT	TTGCAGTTGCCGGAACTAAT	50.0	45.0	58.4	58.1	525-707 bp	1.0	78.5