Hotspot

rbcL-accD

LSC · 53550-54297 · 748 bp

Back to Metagentiana

Pi 0.0109

MarkerSeek score 58.20

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 748
variable_sites: 8
pi: 0.0109
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GTGGATACGTTGGATCCCAC	CACACCTGCGTCCTAACTTT	55.0	50.0	58.1	58.1	822-830 bp	1.0	80.9
rbcL-accD_p2	TGGATACGTTGGATCCCACT	CACACCTGCGTCCTAACTTT	50.0	50.0	58.1	58.1	821-829 bp	1.0	80.3
rbcL-accD_p3	AGTGGATACGTTGGATCCCA	CACACCTGCGTCCTAACTTT	50.0	50.0	58.1	58.1	823-831 bp	1.0	80.2
rbcL-accD_p4	GTGGATACGTTGGATCCCAC	TTATTTAGCCCACACCTGCG	55.0	50.0	58.1	58.3	832-840 bp	1.0	79.8
rbcL-accD_p5	CAGTGGATACGTTGGATCCC	CACACCTGCGTCCTAACTTT	55.0	50.0	57.8	58.1	824-832 bp	1.0	79.3