Hotspot

atpB-rbcL

LSC · 51325-52091 · 767 bp

Back to Metagentiana

Pi 0.00946

MarkerSeek score 67.60

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 767
variable_sites: 7
pi: 0.00946
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ATGATTTGGACGATACGCCC	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.1	58.0	896-923 bp	1.0	73.9
atpB-rbcL_p2	ATGATTTGGACGATACGCCC	ACCAGCTTTGAATCCAACACT	50.0	42.9	58.1	58.1	895-922 bp	1.0	73.6
atpB-rbcL_p3	GGCGGAAAGGCTACATCTAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	57.8	58.0	926-953 bp	1.0	73.5
atpB-rbcL_p4	GGCGGAAAGGCTACATCTAG	ACCAGCTTTGAATCCAACACT	55.0	42.9	57.8	58.1	925-952 bp	1.0	73.2
atpB-rbcL_p5	CGATACGCCCTAGGGTTTTT	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.7	58.0	886-913 bp	1.0	72.3