Hotspot

trnC-GCA-petN

LSC · 26304-26845 · 542 bp

Back to Metagentiana

Pi 0.00565

MarkerSeek score 62.70

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 542
variable_sites: 3
pi: 0.00565
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	605-786 bp	1.0	80.9
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	599-780 bp	1.0	79.4
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.4	58.0	623-804 bp	1.0	78.7
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	TAGTCCACTTCTTCCCCACA	50.0	50.0	58.1	57.6	658-839 bp	1.0	77.6
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	TCCACTTCTTCCCCACACTA	50.0	50.0	58.1	57.6	655-836 bp	1.0	77.6