Hotspot

trnR-UCU-atpA

LSC · 8175-8297 · 123 bp

Back to Metagentiana

Pi 0.0244

MarkerSeek score 66.50

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 123
variable_sites: 3
pi: 0.0244
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	ACGAATGAAAAGCGTCCATTG	AAGACATTTACCGAGGAGGC	42.9	50.0	57.8	57.3	293-294 bp	1.0	65.7
trnR-UCU-atpA_p2	CGAATGAAAAGCGTCCATTGT	AAGACATTTACCGAGGAGGC	42.9	50.0	57.8	57.3	292-293 bp	1.0	65.7
trnR-UCU-atpA_p3	ACGAATGAAAAGCGTCCATTG	GAGGCGCAAATCCTTTTGAA	42.9	45.0	57.8	57.3	278-279 bp	1.0	65.5
trnR-UCU-atpA_p4	CGAATGAAAAGCGTCCATTGT	GAGGCGCAAATCCTTTTGAA	42.9	45.0	57.8	57.3	277-278 bp	1.0	65.5
trnR-UCU-atpA_p5	CGAATGAAAAGCGTCCATTGT	GAGGAGGCGCAAATCCTTTT	42.9	50.0	57.8	58.8	280-281 bp	1.0	64.7