Hotspot

ndhF

SSC · 2-2224 · 2223 bp

Back to Mesoamerantha

Pi 0.0162

MarkerSeek score 53.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2223
variable_sites: 36
pi: 0.0162
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGAACTTGAATGTAAAAGATTTGT	GTATCCTCACGAATCGGACA	25.0	50.0	53.4	56.9	166 bp	1.0	42.4
ndhF_p2	AGAACTTGAATGTAAAAGATTTGT	TGTATCCTCACGAATCGGAC	25.0	50.0	53.4	56.9	167 bp	1.0	42.4
ndhF_p3	AGAACTTGAATGTAAAAGATTTGT	ATCCTCACGAATCGGACAAT	25.0	45.0	53.4	56.7	164 bp	1.0	42.4
ndhF_p4	AGAACTTGAATGTAAAAGATTTGT	CCATGTATCCTCACGAATCGG	25.0	52.4	53.4	58.3	170 bp	1.0	42.4
ndhF_p5	AGAACTTGAATGTAAAAGATTTGT	GTATCCTCACGAATCGGACAA	25.0	47.6	53.4	57.6	166 bp	1.0	42.4