Hotspot

ndhA

SSC · 9998-12149 · 2152 bp

Back to Mesoamerantha

Pi 0.0149

MarkerSeek score 53.50

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2152
variable_sites: 32
pi: 0.0149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CGGGCATACACGAACACATA	AGCCCCAAAAGGAGAATTGG	50.0	50.0	58.1	58.1	2654-2656 bp	1.0	80.1
ndhA_p2	CGGGCATACACGAACACATA	TTCGTCCACCCGGTTTTATC	50.0	50.0	58.1	57.9	2586-2588 bp	1.0	79.8
ndhA_p3	CGGGCATACACGAACACATA	TGGGAAGTCCAATGGCAAAA	50.0	45.0	58.1	57.9	2908-2910 bp	1.0	79.7
ndhA_p4	ATCGACCCAAAGCGATTTGA	AGCCCCAAAAGGAGAATTGG	45.0	50.0	57.9	58.1	2862-2864 bp	1.0	79.7
ndhA_p5	TCGACCCAAAGCGATTTGAT	AGCCCCAAAAGGAGAATTGG	45.0	50.0	57.9	58.1	2861-2863 bp	1.0	79.7