Hotspot

atpF

LSC · 58221-59615 · 1395 bp

Back to Mesoamerantha

Pi 0.0138

MarkerSeek score 54.60

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1395
variable_sites: 19
pi: 0.0138
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CGCTCCTGGAAAGTAGTCAC	ATTTCTGCCGCTTCCGTTAT	55.0	45.0	58.0	58.0	2534-2818 bp	1.0	81.3
atpF_p2	CGCTCCTGGAAAGTAGTCAC	GTTGTGGCATTAGCGCTTTT	55.0	45.0	58.0	57.9	2342-2626 bp	1.0	81.2
atpF_p3	ATGAATACGAGCAATGCCGT	ATTTCTGCCGCTTCCGTTAT	45.0	45.0	58.0	58.0	2016-2300 bp	1.0	81.2
atpF_p4	CGCTCCTGGAAAGTAGTCAC	GGCGAGCGAAGTGATAGAAA	55.0	50.0	58.0	58.1	2185-2200 bp	1.0	81.1
atpF_p5	CGCTCCTGGAAAGTAGTCAC	GAGACAACCAGAAGCAGAGG	55.0	55.0	58.0	57.9	2427-2711 bp	1.0	80.9