Hotspot

psaJ-rpl33

LSC · 116105-116623 · 519 bp

Back to Mesoamerantha

Pi 0.0179

MarkerSeek score 70.50

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 519
variable_sites: 9
pi: 0.0179
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTGGGTCTTTAGCGGGTCTA	CCCGCGATTCCTTATTGACA	50.0	50.0	58.1	58.1	668-684 bp	1.0	82.2
psaJ-rpl33_p2	TATTCCCAGATGCCCTGTCA	CCCGCGATTCCTTATTGACA	50.0	50.0	58.1	58.1	629-645 bp	1.0	82.0
psaJ-rpl33_p3	CGTGGCACCTGTGTTAACTA	CCCGCGATTCCTTATTGACA	50.0	50.0	57.8	58.1	697-713 bp	1.0	81.3
psaJ-rpl33_p4	ATGGTTTGGGTCTTTAGCGG	CCCGCGATTCCTTATTGACA	50.0	50.0	58.2	58.1	673-689 bp	1.0	81.3
psaJ-rpl33_p5	TGGGTCTTTAGCGGGTCTAT	CCCGCGATTCCTTATTGACA	50.0	50.0	57.8	58.1	667-683 bp	1.0	81.3