Hotspot

ndhC-trnV-UAC

LSC · 98245-99340 · 1096 bp

Back to Mesoamerantha

Pi 0.0116

MarkerSeek score 70.00

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1096
variable_sites: 12
pi: 0.0116
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CTAACCGGGGCTAAAACTCC	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.0	57.8	1193-1233 bp	1.0	82.9
ndhC-trnV-UAC_p2	CGGGGCTAAAACTCCTGAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.8	57.8	1188-1228 bp	1.0	81.7
ndhC-trnV-UAC_p3	TAACCGGGGCTAAAACTCCT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.3	57.8	1192-1232 bp	1.0	80.5
ndhC-trnV-UAC_p4	CTAACCGGGGCTAAAACTCC	GAAGGTCTACGGTTCGAGTC	55.0	55.0	58.0	57.5	1192-1232 bp	1.0	80.2
ndhC-trnV-UAC_p5	TCTCGGGTCCTTCACTAACC	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.4	57.8	1207-1247 bp	1.0	79.6