Hotspot

rpl32-trnL-UAG

SSC · 3493-4280 · 788 bp

Back to Mesoamerantha

Pi 0.0218

MarkerSeek score 71.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 788
variable_sites: 17
pi: 0.0218
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CACCGGGCATTCAAAAAGTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.5	58.0	852-860 bp	1.0	78.1
rpl32-trnL-UAG_p2	CACCGGGCATTCAAAAAGTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.5	58.0	853-861 bp	1.0	78.1
rpl32-trnL-UAG_p3	CACCGGGCATTCAAAAAGTT	TTTAGAAGATGCCGCCACTC	45.0	50.0	57.5	58.0	912-920 bp	1.0	77.8
rpl32-trnL-UAG_p4	TTCCACCGGGCATTCAAAAA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.6	58.0	855-863 bp	1.0	77.6
rpl32-trnL-UAG_p5	TTTCCACCGGGCATTCAAAA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.6	58.0	856-864 bp	1.0	77.6