Hotspot

ndhF-rpl32

SSC · 2225-3333 · 1109 bp

Back to Mesoamerantha

Pi 0.0268

MarkerSeek score 65.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1109
variable_sites: 29
pi: 0.0268
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAAGAAAAGGTATTATCCACGC	GGTGGAAACCGATTTCGCTA	43.5	50.0	57.6	58.3	1266-1273 bp	1.0	53.9
ndhF-rpl32_p2	AGAAAAGGTATTATCCACGCA	GGTGGAAACCGATTTCGCTA	38.1	50.0	55.0	58.3	1263-1270 bp	1.0	49.4
ndhF-rpl32_p3	GAAGAAAAGGTATTATCCACGCA	GGTGGAAACCGATTTCGCTA	39.1	50.0	56.9	58.3	1265-1272 bp	1.0	48.8
ndhF-rpl32_p4	AAGAAAAGGTATTATCCACGCA	GGTGGAAACCGATTTCGCTA	36.4	50.0	55.8	58.3	1264-1271 bp	1.0	47.7
ndhF-rpl32_p5	GTGGAAGAAAAGGTATTATCCACG	GGTGGAAACCGATTTCGCTA	41.7	50.0	57.6	58.3	1268-1275 bp	1.0	46.4