Hotspot

ycf1

SSC · 124352-130081 · 5730 bp

Back to Mesembryanthemum

Pi 0.0121

MarkerSeek score 46.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5730
variable_sites: 69
pi: 0.0121
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 46.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCGATATGGGACGGCTTAGT	TGAATCATCCATTCAAGCCCA	50.0	42.9	58.0	57.6	1448-1451 bp	1.0	70.3
ycf1_p2	GATAGGGATCCTCCACCACA	TGAATCATCCATTCAAGCCCA	55.0	42.9	57.9	57.6	1138-1141 bp	1.0	69.7
ycf1_p3	GTGATAGGGATCCTCCACCA	TGAATCATCCATTCAAGCCCA	55.0	42.9	57.9	57.6	1140-1143 bp	1.0	69.7
ycf1_p4	TGATAGGGATCCTCCACCAC	TGAATCATCCATTCAAGCCCA	55.0	42.9	57.9	57.6	1139-1142 bp	1.0	69.7
ycf1_p5	CAGATTCGATATGGGACGGC	TGAATCATCCATTCAAGCCCA	55.0	42.9	58.3	57.6	1453-1456 bp	1.0	68.1