Hotspot

ndhE-ndhG

SSC · 118583-118824 · 242 bp

Back to Mesembryanthemum

Pi 0.0395

MarkerSeek score 70.60

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 242
variable_sites: 9
pi: 0.0395
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	TTTCGGCTCGTGACTAATCC	TTGCTGTGGCTCGTCAATAA	50.0	45.0	57.7	57.8	326-339 bp	1.0	81.8
ndhE-ndhG_p2	TTTCGGCTCGTGACTAATCC	ATTGCTGTGGCTCGTCAATA	50.0	45.0	57.7	57.6	327-340 bp	1.0	80.0
ndhE-ndhG_p3	TTTCGGCTCGTGACTAATCC	AATTGCTGTGGCTCGTCAAT	50.0	45.0	57.7	58.5	328-341 bp	1.0	79.5
ndhE-ndhG_p4	TCGGCTCGTGACTAATCCAT	TTGCTGTGGCTCGTCAATAA	50.0	45.0	58.6	57.8	324-337 bp	1.0	79.3
ndhE-ndhG_p5	CAAGACACATAAGAGCCCGA	TTGCTGTGGCTCGTCAATAA	50.0	45.0	57.3	57.8	352-365 bp	1.0	78.3