Hotspot

rpl20-rps12

LSC · 70230-71007 · 778 bp

Back to Mesembryanthemum

Pi 0.0142
MarkerSeek score 67.40
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
778
variable_sites
11
pi
0.0142
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 CCCTCGAAAGCTTGATCCAA CGCCGAGGAACATGTACTAG 50.0 55.0 57.9 57.9 879-883 bp 1.0 79.5
rpl20-rps12_p2 CTTGAATGAGCCCCTCGAAA CGCCGAGGAACATGTACTAG 50.0 55.0 57.9 57.9 890-894 bp 1.0 79.5
rpl20-rps12_p3 TTGAATGAGCCCCTCGAAAG CGCCGAGGAACATGTACTAG 50.0 55.0 57.9 57.9 889-893 bp 1.0 79.5
rpl20-rps12_p4 CCCTCGAAAGCTTGATCCAA GCCGAGGAACATGTACTAGG 50.0 55.0 57.9 57.5 878-882 bp 1.0 76.4
rpl20-rps12_p5 TTGAATGAGCCCCTCGAAAG GCCGAGGAACATGTACTAGG 50.0 55.0 57.9 57.5 888-892 bp 1.0 76.4