Hotspot

rps18-rpl20

LSC · 69561-69842 · 282 bp

Back to Mesembryanthemum

Pi 0.0208

MarkerSeek score 66.20

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 282
variable_sites: 5
pi: 0.0208
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	CGAGTCGACTACTAGAACTGC	GAAAGAATCCCCCGAAACGA	52.4	50.0	57.7	57.9	321-359 bp	1.0	73.9
rps18-rpl20_p2	CGAGTCGACTACTAGAACTGC	AAAGAATCCCCCGAAACGAT	52.4	45.0	57.7	57.2	320-358 bp	1.0	68.3
rps18-rpl20_p3	CGAGTCGACTACTAGAACTGC	AAGAATCCCCCGAAACGATT	52.4	45.0	57.7	57.2	319-357 bp	1.0	68.3
rps18-rpl20_p4	CGAGTCGACTACTAGAACTGC	AGAATCCCCCGAAACGATTT	52.4	45.0	57.7	57.2	318-356 bp	1.0	68.3
rps18-rpl20_p5	CGAGTCGACTACTAGAACTGC	GGAAAGAATCCCCCGAAACG	52.4	55.0	57.7	58.9	322-360 bp	1.0	67.5