Hotspot

trnF-GAA-ndhJ

LSC · 49358-49993 · 636 bp

Back to Mesembryanthemum

Pi 0.0288

MarkerSeek score 68.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 636
variable_sites: 18
pi: 0.0288
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	CCCGAAAGTTGGGTAGGATG	55.0	55.0	58.0	58.0	784-815 bp	1.0	82.5
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	CCGAAAGTTGGGTAGGATGG	55.0	55.0	58.0	58.0	783-814 bp	1.0	82.5
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	CCCGAAAGTTGGGTAGGATG	50.0	55.0	58.1	58.0	748-779 bp	1.0	82.2
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	CCGAAAGTTGGGTAGGATGG	50.0	55.0	58.1	58.0	747-778 bp	1.0	82.2
trnF-GAA-ndhJ_p5	AAAGGGGAATGGTCGGGATA	CCGAAAGTTGGGTAGGATGG	50.0	55.0	58.1	58.0	797-828 bp	1.0	81.7