Hotspot

psaA-ycf3

LSC · 42477-43296 · 820 bp

Back to Mesembryanthemum

Pi 0.0237

MarkerSeek score 74.60

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 820
variable_sites: 19
pi: 0.0237
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GGGGCCCTTAGCTATTGTTC	ATCAAGCCGCTGAGTATTGG	55.0	50.0	58.0	58.0	1028-1038 bp	1.0	82.5
psaA-ycf3_p2	GGGGCCCTTAGCTATTGTTC	ATTGGTTGAAGATCACGGGG	55.0	50.0	58.0	57.9	962-972 bp	1.0	82.0
psaA-ycf3_p3	GGGGCCCTTAGCTATTGTTC	CGATCAAGCCGCTGAGTATT	55.0	50.0	58.0	58.1	1030-1040 bp	1.0	81.7
psaA-ycf3_p4	AAAAATGACCGGGTCTAGCC	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	1005-1015 bp	1.0	81.7
psaA-ycf3_p5	AAAAATGACCGGGTCTAGCC	ATTGGTTGAAGATCACGGGG	50.0	50.0	57.9	57.9	939-949 bp	1.0	81.2