Hotspot

trnE-UUC-trnT-GGU

LSC · 31480-32035 · 556 bp

Back to Mesembryanthemum

Pi 0.0144

MarkerSeek score 64.40

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 556
variable_sites: 8
pi: 0.0144
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	659-660 bp	1.0	78.8
trnE-UUC-trnT-GGU_p2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	638-639 bp	1.0	78.7
trnE-UUC-trnT-GGU_p3	GCTGCCTCCTTGAAAGAGAG	CCGATGACTTACGCCTTACC	55.0	55.0	58.3	58.2	656-657 bp	1.0	77.7
trnE-UUC-trnT-GGU_p4	TGTCCTGAACCACTAGACGA	CCGATGACTTACGCCTTACC	50.0	55.0	57.7	58.2	635-636 bp	1.0	77.5
trnE-UUC-trnT-GGU_p5	GCTGCCTCCTTGAAAGAGAG	TTGAACCGATGACTTACGCC	55.0	50.0	58.3	58.3	661-662 bp	1.0	76.6